Analysis Note

ControlHuman pancreas tissue lysate

Application

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected RIP3 in 10 µg of human placenta tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RIP3 in rat pancreas tissue.

Research CategoryApoptosis & Cancer

This Anti-RIP3 Antibody, clone 6E6.2 is validated for use in WB, IH for the detection of RIP3.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Receptor-interacting protein 3 (RIP3) contains a N-terminal kinase domain and interacts with RIP. The binding of RIP to RIP3 causes the RIP3/RIP complex to be shuttled into the tumor necrosis factor receptor-1 signaling cascade. RIP3 is known to be a very effective apoptosis inducer. Two novel splice variants of RIP3 have been discovered, RIP3 beta and RIP3 gamma, that have shown to decrease apoptosis related to full length RIP3. RIP3 gamma has been shown to be significantly up-regulated in colon and lung cancers and may play a role in tumorigenesis.

Immunogen

GST-tagged recombinant protein corresponding to human RIP3 at the N-terminus.

Epitope: N-terminus

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human pancreas tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected RIP3 in 10 µg of human pancreas tissue lysate.

Specificity

This antibody recognizes RIP3 at the N-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~56 kDa (isoform 1) and ~27 kDa (isoform 2) observed.
Isoform 3 (~25 kDa) may be observed in some lysates. An uncharacterized band may be observed at ~16 kDa in some lysates.